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A , UMAP visualization of drug sensitivity profiles for 528 compounds tested across 43 ALL cell lines. Each point represents a compound, positioned based on similarity of killing profiles. B , sDSS values for triciribine and PI3K-Akt-mTOR pathway inhibitors across 43 ALL cell lines. The dashed line indicates sDSS = 8. The mean sDSS value for each inhibitor is indicated by an ‘×’ or a larger dot. Akt inhibitors are indicated in red. C , Pairwise Spearman correlation analysis of drug sensitivity profiles for PI3K-Akt-mTOR pathway inhibitors across 43 ALL cell lines. Akt inhibitors are indicated in red. D , Immunoblot analysis of phosphorylated Akt (Ser473), total Akt, and the loading control <t>SOD1</t> in ALL-SIL and P12-ICHIKAWA cells following treatment with indicated concentrations or durations of triciribine.
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A , UMAP visualization of drug sensitivity profiles for 528 compounds tested across 43 ALL cell lines. Each point represents a compound, positioned based on similarity of killing profiles. B , sDSS values for triciribine and PI3K-Akt-mTOR pathway inhibitors across 43 ALL cell lines. The dashed line indicates sDSS = 8. The mean sDSS value for each inhibitor is indicated by an ‘×’ or a larger dot. Akt inhibitors are indicated in red. C , Pairwise Spearman correlation analysis of drug sensitivity profiles for PI3K-Akt-mTOR pathway inhibitors across 43 ALL cell lines. Akt inhibitors are indicated in red. D , Immunoblot analysis of phosphorylated Akt (Ser473), total Akt, and the loading control <t>SOD1</t> in ALL-SIL and P12-ICHIKAWA cells following treatment with indicated concentrations or durations of triciribine.
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A , UMAP visualization of drug sensitivity profiles for 528 compounds tested across 43 ALL cell lines. Each point represents a compound, positioned based on similarity of killing profiles. B , sDSS values for triciribine and PI3K-Akt-mTOR pathway inhibitors across 43 ALL cell lines. The dashed line indicates sDSS = 8. The mean sDSS value for each inhibitor is indicated by an ‘×’ or a larger dot. Akt inhibitors are indicated in red. C , Pairwise Spearman correlation analysis of drug sensitivity profiles for PI3K-Akt-mTOR pathway inhibitors across 43 ALL cell lines. Akt inhibitors are indicated in red. D , Immunoblot analysis of phosphorylated Akt (Ser473), total Akt, and the loading control <t>SOD1</t> in ALL-SIL and P12-ICHIKAWA cells following treatment with indicated concentrations or durations of triciribine.
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A , UMAP visualization of drug sensitivity profiles for 528 compounds tested across 43 ALL cell lines. Each point represents a compound, positioned based on similarity of killing profiles. B , sDSS values for triciribine and PI3K-Akt-mTOR pathway inhibitors across 43 ALL cell lines. The dashed line indicates sDSS = 8. The mean sDSS value for each inhibitor is indicated by an ‘×’ or a larger dot. Akt inhibitors are indicated in red. C , Pairwise Spearman correlation analysis of drug sensitivity profiles for PI3K-Akt-mTOR pathway inhibitors across 43 ALL cell lines. Akt inhibitors are indicated in red. D , Immunoblot analysis of phosphorylated Akt (Ser473), total Akt, and the loading control SOD1 in ALL-SIL and P12-ICHIKAWA cells following treatment with indicated concentrations or durations of triciribine.

Journal: bioRxiv

Article Title: Proteomic profiling reveals pleiotropic antimetabolite activity of triciribine in acute lymphoblastic leukemia

doi: 10.64898/2026.03.13.710465

Figure Lengend Snippet: A , UMAP visualization of drug sensitivity profiles for 528 compounds tested across 43 ALL cell lines. Each point represents a compound, positioned based on similarity of killing profiles. B , sDSS values for triciribine and PI3K-Akt-mTOR pathway inhibitors across 43 ALL cell lines. The dashed line indicates sDSS = 8. The mean sDSS value for each inhibitor is indicated by an ‘×’ or a larger dot. Akt inhibitors are indicated in red. C , Pairwise Spearman correlation analysis of drug sensitivity profiles for PI3K-Akt-mTOR pathway inhibitors across 43 ALL cell lines. Akt inhibitors are indicated in red. D , Immunoblot analysis of phosphorylated Akt (Ser473), total Akt, and the loading control SOD1 in ALL-SIL and P12-ICHIKAWA cells following treatment with indicated concentrations or durations of triciribine.

Article Snippet: Primary antibodies included ADK (AM8619b-ev; Nordic Biosite), SOD1 (sc-17767; Santa Cruz Biotechnology), PARP (9532), Akt (4691), and phospho-Akt (Ser473; 4060) (Cell Signaling Technology).

Techniques: Western Blot, Control